Researchers can use the RealBio D4™ Culture System to achieve reliable culture samples without the need to use xenograph models. Because the cells in our system organize closely mimic the natural function of analogous tissue in vivo, researchers have a distinct advantage over those using traditional methods, including most other so-called 3D cell culture technologies.

The RealBio D4™ Culture System was originally designed for the culture of human bone marrow for early progenitor stem cells that may be continually harvested. Because a range of hematopoietic cells of varying degrees of differentiation are continually “shed” into the circulating nutrient stream in a manner similar to how the marrow supplies cells to the blood in vivo, the cells that migrate and release into the culture medium are easily harvested for use in a variety of research applications.

Because the RealBio D4™ Culture System provides a structure that allows gas and nutrients to diffuse through the cultured tissues more like within tissues in vivo, researchers can attain more biorelevant results than traditional in vitro systems for evaluation of toxicity or the questions around ADME testing of new agents for either drug development, nutraiceuticals testing or chemical safety evaluations. The system enables more rapid feedback regarding potential treatment options than possible using patients in a clinical setting, and enables evaluation of compounds and variables that cannot be ethically tested on humans or in other live-animal models.

The RealBio D4™ Culture System has also shown promise in the bioproduction of cell and tissue growth and cultures. It has demonstrated increased production of antibodies with a reduction of input energy. In one study completed at the University of Michigan, the RealBio D4™ Culture System produced more than three times the quantity of a monoclonal antibody over a 30-day period, using only a quarter of the resource media used in the Integra CL1000 culture flask.